Histology
The University of Queensland provides histology facilities via the Queensland Brain Institute, School of Biomedical Sciences and Translational Research Institute. Services include sample preparation and staining, as well as sectioning and processing.
Services
- Tissue processing and sectioning
- Paraffin and resin embedding
- Histochemical staining including silver stains, Luxol Fast Blue, Cresyl Violet (Nissl), Golgi Cox and Hematoxylin and Eosin
- Antigen recovery
- Immunohistochemical staining and Tissue clearing including Clarity and IDISCO, Cubic, SHEILD
Equipment
- 2 x Leica RM2235 manual rotary microtomes
- Thermofisher HM325 manual rotary microtome
- Leica EM Uc6 ultramicrotome
- Leica EM KMR2 glass knife maker
- 3 x Leica Vibratomes VT1000S
- Leica SM2010R Sliding microtome
- Thermofisher Cryostar NX70 cryostat
- Micron HM560 cryostat
- Olympus SZ51 dissecting stereo microscope
- Olympus cx31 multiheaded teaching microscope
- Biocare Medical antigen recovery pressure cooker
- Leica TP1020 paraffin tissue processor
- Medite TES Valida paraffin embedding station
- Pelco Biowave Pro microwave tissue processor
- Primera Signature slide and cassette printers
- Tissue clearing equipment
- Smart Clear II Pro, life Canvas Technologies
- Smart label life Canvas Technologies
- EasyGel Module
Contact
Dr Robert Sullivan
Histology Facility Manager
Website: Queensland Brain Institute
Phone: 07 3346 6300
Email: qbi.histology@uq.edu.au
Location: St Lucia campus
Services
Self, assisted and full service:
- Specialised systems for tissue preparation
- Slide production
- Microtomy
- Vibratomy
- Histo-chemical staining
- Immunohistochemistry
Equipment
- Dakewe HP300 Automated Tissue Processor
- Leica HistoCore Arcadia H Embedding Station
- Leica Semi-Automated RM2245 Rotary Microtomes (x2)
- Microm HM340E Rotary Microtome
- Hyrax M25 Rotary Microtome
- Microm HM430 Sliding Microtome
- Leica VT1000S Vibrating Blade Microtome
- Cryostar NX70 Cryostats (x2)
- Histochemical Staining Facility
- BioCare Medical NxGen Decloaking Chamber
- Epredia SlideMate AS Slide Printer (x2)
- Epredia PrintMate AS Cassette Printer
- Nikon Eclipse 50i Light Microscope with Camera
- Nikon Eclipse Ci Light Microscope with Camera
- Nikon SMZ745T Stereomicroscope with Camera (x2)
Contact
Dr Darryl Whitehead
Manager
Website: School of Biomedical Sciences
Phone: 07 3365 2725
Email: biomedhistology@uq.edu.au
Location: St Lucia campus
Services
TRI supports both full service and self service histology. It provides high quality instrumentation and a wide range of services such as tissue processing, microtomy, cryomicrotomy, histo-chemical staining, immunohistochemistry, immunofluorescence and multiplexing. The facility can prepare frozen and formalin fixed samples as well as process the samples through sectioning, including for specialised spatial requirements, and various staining techniques suitable for research use.
TRI provides training and access to specialised equipment for tissue preparation, slide production and histochemical staining for researchers and students.
Self and full service:
- Tissue processing
- Microtomy
- Cryomicrotomy
- Histochemical staining
- Immunohistochemistry
- Tissue Micro Array (TMA) production
- Immunofluorescence/Multiplexing
- DNAse/RNAse-free paraffin sections
Equipment
- Leica Embedding station
- Leica Microtomes
- Cryostats (including bone sectioning capability)
- Automated tissue processor
- Slide printer
- Cassettes Printer
- Automated stainer and cover slipper
- Ventana Automated IHC system to Ventana Automated IHF/IF system
- Automated Tissue Microarrayer
The majority of equipment is TRI owned.
Contact
Website: TRI Histology Facility
Email: histologycore@tri.edu.au
Location: Princess Alexandra Hospital campus
Services
SVS supports both full service and self-service histology. It provides high quality instrumentation and a wide range of services such as tissue processing, microtomy, histo-chemical staining and immunohistochemistry. The facility can formalin fix samples as well as process existing samples through sectioning and various staining techniques suitable for research use.
SVS provides training and access to specialised equipment for tissue preparation, slide production and histochemical staining for researchers and students.
The lab also has an extensive sample collection of embedded blocks of tissue that is all referenced and reported on through a research database. The school has a team of Pathologists and Residents that are willing to collaborate with other researchers on historical and new samples and draw upon the extensive dataset.
Self and full service:
- Tissue processing and sectioning
- Paraffin embedding
- Microtomy
- Histochemical staining
Equipment
- Sakura Tissue Tek VIP®6 AI-E2
- Sakura Hito-Tek® VIP1™
- Leica EG1160 Embedding station
- Leica RM2255 rotary microtome
- Leica RM2235 manual rotary microtome
- Microtec CUT 4055 manual rotary microtome
- Cassettes Printer
- Automated stainer and cover slipper
- Dako LINK48 Automated IHC system
Contact
Website: Veterinary Laboratory Services
Email: vis@uq.edu.au
Location: Gatton campus