Flow cytometry
Flow cytometry is used to isolate and quantify specific cell populations and provide quantitative information pertaining to viability, DNA content, mitotic activity and protein expression patterns of specific cell types.
Queensland Brain Institute (QBI)
Services
Flow Cytometry (FC) is used to rapidly detect, identify, and quantify specific cell populations. FC provides quantitative information pertaining to viability, DNA content, mitotic activity and protein expression patterns of specific cell types. FC has applications in all fields of science where cells or particles can be placed in a single cell suspension. The FC core facility at QBI offers training, experimental design and analysis on analytical flow cytometry and cell sorting, and is adaptable to new applications and methodologies.
Equipment
• BD FACSymphony™ S6 Cell Sorter- 5 Laser (UV 355nm, Violet 405nm, Blue 488nm, Yellow/Green 562nm & Red 633nm), 28 fluorescent parameters, 6-way sorting, index tray sorting, differing nozzle sizes to meet different sample types, housed in a ClassII biological cabinet.
•BD FACS AriaI™ Cell Sorter- 3 Laser (Violet 405nm, Blue 488nm & Red 633nm), 14 fluorescent parameters, 4-way sorting, multi well plate deposition, differing nozzle sizes for all sample types.
•BD Influx™ Cell Sorter- 5 Laser (UV 355nm, Violet 405nm, Blue 488nm, Yellow/Green 561nm & Red 633nm), 14 fluorescent parameters, 4-way sorting, index tray sorting, differing nozzle sizes for diverse cell types, house in a ClassI biological cabinet.
•BD FACSymphony™A5 SE Analytical Flow Cytometer- 5 Laser (UV 355nm, Violet 405nm, Blue 488nm, Yellow/Green 561nm & Red 633nm), 50 fluorescent parameters, choose between spectral or compensation-based cell analysis, equipped with a high throughput sampler (HTS) option for fully automated sample acquisition.
•BD LSRII™ Analytical Flow Cytometer- 5 Laser (UV 355nm, Violet 405nm, Blue 488nm, Yellow/Green 562nm & Red 633nm), 18 fluorescent parameters.
Contact
Flow Cytometry Facility Manager
Virginia Nink
(07) 3346 6378
School of Biomedical Sciences (SBMS)
Services
Flow cytometry capability.
Equipment
- BD Fortessa Flow Cytometer - FlowJo 10 software
Multicolour cell analyser with four lasers allow you to analyse up to 15 different parameters at once from fluorescently labelled samples. Our remote access computing platform allows you to analyse even the most complex datasets from anywhere in the world with internet access using FlowJo software.
Contact
Dr Shaun Walters
(07) 3365 1754
School of Biomedical Sciences, Level 2 Skerman Building (65), St Lucia campus
School of Chemistry and Molecular Biosciences (SCMB)
Services
The SCMB Flow Cytometer Facility offers access, training and help with experiment design and data analysis for analytical flow cytometry and fluorescence activated cell sorting (FACS). The Facility specialises on sorting and analysis of environmental samples, bacteria and yeast cells.
Equipment
- BD-FACS Aria II high speed cell sorter - 3 lasers UV (355nm), Blue (488nm), Yellow-Green (561nm); 12 detectors. Sorting into tubes (up to 4 populations), multiwell plates or onto glass slides.
- BD-AccuriC6 and BD-AccuriCSampler analytical flow cytometers - 2 lasers: Blue (488nm) and Red (640 nm)– 4 detectors. Automated data acquisition form 24-tube rack or multiwall plates.
- BC-CytoFlexS flow cytometers. Two laser configurations: Violet (405nm with SSC detector for small particles), Blue (488nm), Yellow Green (5Y61nm) and Blue (488nm), Yellow Green (5Y61nm), Red (640nm) – up to 10 detectors.
- Analysis computer with FlowJo, CytExpert and remote access to FACSDiva software.
Contact
Dr Vitaliya Sagulenko
School of Chemistry and Molecular Biosciences
(07) 3365 7539 or 0409 132 834
Translational Research Institute (TRI)
Services
Providing assistance in multi-parameter experimental design, instrument operation and quality control, and full in-house training in all areas of flow cytometry. In addition, the Flow Cytometry Core offers high-speed cell sorting into tubes (up to 6 populations at once) and plates.
Equipment
The following equipment is available to all users as part of the 'Core Facilities at TRI'.
- Beckman Coulter Gallios (3 laser 9 colour) analysers
- Becton Dickinson Fortessa X-20 (5 laser 18 colour) analysers
- Becton Dickinson Accuri C6 (2 laser 4 colour) analyser
- Beckman Coulter Cytoflex S (4 laser 13 colour) analysers
- Merck-Millipore Imagestreamx Mark II Imaging cytometer
- Mindray BC-5000 veterinary haematology analyser
- BD FACSymphony A5 ultra-high parameter analyser
- Beckman Coulter MoFlo Astrios EQ(7 laser 24 colour) Sorter
- BD FACSAria Fusion (5 laser 18 colour) Sorters
- Miltenyi Biotex Automacs Pro Cell Separator
- Suite of Analysis computers and software
(The majority of equipment is TRI owned)
Contact
Translational Research Institute (TRI)
Level 4, TRI Building (48), Princess Alexandra Hospital campus
- Analytical and environmental
- Biological resources
- Computation
- Crystallography
- Flow cytometry
- Genomics
- Histology
- Humanities and Business
- Hypersonics
- Imaging
- Mass Spectrometry
- Materials and Fabrication
- Microscopy
- Microwave Engineering and Photonics
- Mining and minerals processing
- Nanofabrication
- Nuclear magnetic resonance (NMR)
- Recombinant protein production
- Research Stations
- Spectroscopy
Get in touch
For general information about UQ's equipment and infrastructure, contact the Director, Research Infrastructure.
(07) 3443 1033