Mass Spectrometry
Mass Spectrometry has a range of applications, including molecular discovery, identification, characterisation and quantification. The Mass Spectrometry equipment the University of Queensland has on offer is available at the following locations:
- Centre for Microscopy and Microanalysis (CMM)
- Centre for Advanced Imagery (CAI)
- Institute of Molecular Bioscience (IMB)
- School of Chemistry and Molecular Biosciences (SCMB)
- Translational Research Institute (TRI)
Centre for Clinical Research (UQCCR)
Services
The Mass Spectrometry facility at UQ Centre for Clinical Research is located on the Royal Brisbane and Women’s Hospital site at Herston and is NATA accredited for research, development, and evaluation (ISO/ IEC 17025). The MS facility team have extensive experience in a wide range of application areas including the identification of proteins/metabolites/lipids/small molecules, quantitation workflows including labelled and label free (SWATH and MRM), intact protein analysis and characterisation, and lipidomics workflows. The facility also provides training on sample preparation and the use of the specific instruments.
Equipment
- SCIEX TripleTOF 5600 System x 2
- SCIEX QTRAP 5500 System
- SCIEX TOF/TOF 5800 System
- Shimadzu LCMS 8050 System
- Agilent 6490 System
- SelexION® Differential Ion Mobility (DMS)
- Shimadzu Nexera UHPLC Systems
- Eksigent NanoLC 400 Systems
- Eksigent MicroLC 200 System
- Agilent 1290 Infinity
- TriVersa Nanomate System
- Agilent 1100 HPLC systems with UV detector and fractionator
- Hamilton MicroLab® Star Robotic system
- Agilent 3100 Offgel Electrophoresis Fractionator
- Various software solutions for protein identification, quantitation of analytes, intact protein characterisation and lipid analysis.
Contact
Head, Mass Spectrometry Facility
(07) 3346 5007
Centre for Microscopy and Microanalysis (CMM) and Centre for Advanced Imaging (CAI)
Services
Mass Spectrometry Imaging produces the spatial distribution of various analyte classes present in tissue samples mounted on microscope slides. By bringing MSI together with other imaging modalities such as microscopy and MRI, a more holistic picture of biomolecular imaging can be achieved. Within the facility we have a number of MALDI-TOF/TOF systems, and an atmospheric pressure MALDI which can be coupled to QqTOF and QIT analysers. We also now have a JEOL MALDI Spiral-TOF, capable of higher mass resolving power (75,000). Recent addition is a Bruker TIMS TOF Flex with MALDI-2, an instrument capable of both MALDI and Electrospray, making it a very useful instrument for Mass Spectrometry Imaging and companion LC-MS analysis. The broad range of instruments allow us to perform Mass Spectrometry Imaging for a diverse range of analytes. In addition to mass spectrometers, we have a suite of supporting equipment including HPLC-spotting robots, and matrix deposition devices (both spray and vacuum sublimation based. A range of supporting software is also available, most of which can be accessed remotely.
Equipment
- Bruker Autoflex Speed (MALDI-TOF/TOF)
- Burker Ultraflextreme (MALDI-TOF/TOF)
- Bruker MicroTOF Q II (QqTOF)
- Bruker TIMS TOF Flex with MALDI-2 (MALDI and ESI QqTOF with ion mobility)
- JEOL Spiral TOF (MALDI-TOF/TOF)
- Masstech AP-MALDI source
- Thermo nanoHPLC with Proteineer Spotting Robot
- Thermo HPLC with Thermo QqQ MS
- Bruker Imageprep
- Vacuum Sublimation Device
- Sunchrom Matrix Sprayer
- Sunchrom Digester
- Basic histology including microtome for MSI sample prep
- Software available includes Mascot, SCILs Lab, Flex Imaging, ClinProt Tools, PEAKS Studio X, Metaboscape.
Contact
Dr Brett Hamilton
Centre for Microscopy and Microanalysis
(07) 3365 7939
Institute for Molecular Bioscience (IMB)
Services
IMB’s Mass Spectrometry Facility is home to a suite of state-of-the-art mass spectrometry, high-performance liquid chromatography, and robotic instrumentation that have been refined and optimised to investigate biological systems in a high-throughput qualitative and quantitative manner.
The eleven available systems within the facility provide researchers with the resources to investigate a broad range of mass spectrometric applications, including molecular discovery, identification, characterisation and quantification.
Equipment
- SCIEX Triple TOF 6600 and 5600 Electrospray Triple TOF systems
- Agilent 6545 TOF Electrospray QqTOF
- SCIEX 5800 MALDI TOF-TOF
- SCIEX 4000 QTRAP Triple Quadrupole-Linear Ion Trap
- SCIEX 4000 Triple Quadrupole
Contact
Facility Manager
Alun Jones
Institute of Molecular Bioscience
(07) 3346 2996
Institute of Molecular Bioscience, IMB Building (Building 76), St Lucia campus
School of Chemistry and Molecular Biosciences (SCMB)
Services
The SCMB Mass spectrometry facility has a range of instruments for analysis for both biological and synthetic compounds, including proteomics and metabolomics, analysis of natural products, synthetic compounds. Analyses include accurate mass measurement (small molecules and intact proteins), quantitation (SWATH, ITRAQ, SILAC, MRM assays); identification of proteins and other compounds; characterisation of post-translational modifications on proteins (glycosylation, phosphorylation, methylation etc).
Equipment
- Sciex ZenoTOF 7600 QTOF with Waters M-Class UPLC
- 2 x Sciex X500B QTOF with Exion UPLC
- Thermo Orbitrap Elite with ETD with Dionex UltiMate 3000 RSLCnanoHPLC
- Sciex TripleTof 5600 with Shimadzu Prominence nanoHPLC
- Sciex QTRAP 5500 with Shimadzu Nexera UPLC
- Bruker MicrOTof-Q with Dionex UltiMate 3000 HPLC
- Thermo LCQ Fleet ion trap
- ProteinPilot v5.0 software
- ProteomeDiscoverer v4.0 software
- PeakView v2.1 software with SWATH processing script
- ProSight software
- Protein Deconvolution software
- Shimadzu Nexera UPLC system with UV detector
- Agilent 1100/1200 capillary HPLC system
- Sciex OS-Q
- Sciex OS-MQ
- Byonic software
Contact
Dr Amanda Nouwens
School of Chemistry and Molecular Biosciences
(07) 3364 9490
Proteomics - Translational Research Institute (TRI)
Services
The facility is available to research, clinical and industry users from TRI, UQ and the broader community. TRI Proteomics provides expert services in sample analysis, access to specialised equipment, as well as training and assistance.
Equipment
The following equipment is available to all users as part of the 'Core Facilities at TRI'.
- Agilent 3100 Off-gel Electrophoresis (fractionator)
- Bioruptor homogeniser
- Direct detect (protein quantitation)
- Bravo robot (automated sample preparation)
- LC Q-exactive plus Mass Spectrometer
- LC Q-exactive HF Mass Spectrometer
(The majority of equipment is TRI owned)
Contact
Translational Research Institute
Level 5, TRI Building (48), Princess Alexandra Hospital campus
- Analytical and environmental
- Biological resources
- Computation
- Crystallography
- Flow cytometry
- Genomics
- Histology
- Humanities and Business
- Hypersonics
- Imaging
- Mass Spectrometry
- Materials and Fabrication
- Microscopy
- Microwave Engineering and Photonics
- Mining and minerals processing
- Nanofabrication
- Nuclear magnetic resonance (NMR)
- Recombinant protein production
- Research Stations
- Spectroscopy
Get in touch
For general information about UQ's equipment and infrastructure, contact the Director, Research Infrastructure.
(07) 3443 1033