The University of Queensland boasts dedicated research infrastructure facilities specialising in recombinant protein production and stem cell research. Located on UQ’s St Lucia campus, the research enabling facilities below offer highly skilled specialists and equipment.

UQ Protein Expression Facility

The UQ Protein Expression Facility (UQ PEF) is an internationally recognised research facility that specialises in recombinant protein production for academic and industry researchers.

UQ PEF delivers quality proteins for diverse applications in the areas of structural biology and chemistry, drug screening and discovery, vaccine development and delivery, protein therapeutics, functionalised material, biomedical imaging and agricultural science.

The facility offers upskilling programs for research staff and students to develop technical skills in protein production and purification. UQ staff and students can access quality assured materials for molecular cloning, expression and purification via the UQ resource centre on the PEF website. 

Visit the UQ PEF website to learn more about Australia’s most comprehensive range of recombinant protein production services.
 

Protein manufacture - National Biologics Facility

Services

The National Biologics Facility was established through the NCRIS programme to assist academic and industrial researchers to bridge the gap between research discoveries and the bioprocesses required to produce material for pre-clinical studies.  The Facility produces high-quality recombinant proteins, with a focus on biologics for pre-clinical studies. The NBF specialises in use of mammalian cell expression technology for proteins with potential therapeutic or commercial uses.  

Equipment

Activities and services include:

  • Protein and Antibody Engineering
  • Antibody discovery through phage display
  • Molecular Biology
  • Transient expression of proteins in CHO and HEK cells
  • Stable cell line development, creating cell lines suitable for GMP manufacture of product
  • Upstream bioprocessing
  • Downstream bioprocessing
  • Protein purification
  • Protein analytics
  • Access to instrumentation such as Isothermal Colorimetry and Surface Plasmon Resonance

Contact

Doctor Martina Jones

Australian Institute for Bioengineering and Nanotechnology

(07) 3346 3178

Australian Institute for Bioengineering and Nanotechnology, AIBN Building (Building 75), St Lucia campus

Protein preparation and analysis

Services

Facility houses a range of equipment to facilitate sample preparation and analysis of proteins and other biological molecules, including scanning of gels, fractionation / purification of proteins, analysis of protein-protein and other molecular interactions.

Equipment

  • Typhoon FLA7000 phosphor Imager
  • Beckman DTX880 Multimode plate reader
  • Nano-ITC Microcalorimeter
  • GE Ai600 Imager (chemiluminescence, RGB LED fluorescence, UV, Light)
  • Typhoon Variable Mode Scanner (Phospho-storage, chemiluminescence and fluorescence)
  • IPGphor iso-electric focussing units (7, 13 and 24 cm IEF strips)
  • Ettan Dalt II 2nd dimension Electrophoresis System and Gel Caster (24 cm)
  • Progenesis SameSpots software for single and multi-stained gels
  • Waterbath Sonicator
  • Thermo Savant: Speedvac and Freeze Dryer
  • Odyssey Classic Infra-Red laser Imager  
  • Odyssey CLx infra-Red laser Imager
  • SpectraMAX 190 Plate Reader (U/V vis)
  • SpectraMAX 190 Plate Reader 
  • SpectraMAX 250 Plate Reader
  • CLARIOstar Multimode plate reader with environmental control (luminescence, monochromatic fluorescence and absorbance)
  • Cytofluor fluorescence plate reader
  • Eppendorf refrigerated bench top microcentrifuge
  • Umax Image scanner for transmittance scanning of film and gels.
  • Agilent Bioanalyser: for RNA, DNA and protein spectral imaging
  • Microcal iTC200 microcalorimeter
  • Biacore 3000
  • Beckman Multimode detector
  • Beckman Optima XL/I analytical ultracentrifuge
  • AKTA fplc and fraction collector
  • AKTA Pure and fraction collector
  • Beckman Analytical Ultracentrifuge
  • Agilent 1100/1200 capillary HPLC system with UV detector and fraction collector
  • Shimadzu Prominence HPLC system with UV and fluorescence detectors, autosampler and fraction collector
  • Jasco Circular Dichroism (CD) spectrometer 

Contact

Doctor Amanda Nouwens

School of Chemistry and Molecular Biosciences

(07) 3346 9490

Doctor Steven Mason

School of Chemistry and Molecular Biosciences

(07) 3365 4872

Molecular Biosciences Building 76-409, St Lucia campus

Stem cell research

Services

The Pluripotent Stem Cell Core Facility facilitates pluripotent stem cell research both within the University of Queensland and throughout Australia. these services include provision of Human Embryonic Stem Cell Lines (hESCs), Mouse Embryonic Fibroblasts (MEFs), Custom Generated Induced Pluripotent Stem Cells (iPSCs), Genetically Engineered iPSCs Using CRISPRs, Comprehensive Training in Generation and Maintenance of iPSCs, and Mycoplasma, Teratoma & Karyotype Assays.

Equipment

N/A

Contact

Doctor Eva Thoma

Australian Institute for Bioengineering and Nanotechnology

(07) 3365 0339

Australian Institute for Bioengineering and Nanotechnology, AIBN Building 75, St Lucia campus