The University of Queensland offers a wide range of microscopy equipment and analytical instrumentation. Find out what equipment is available from the Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, Centre for Advanced Light Microscopy, Queensland Brain Institute, UQ Diamantina Institute and School of Chemistry and Molecular Biosciences.

Cancer Biology Imaging Facility - Institute for Molecular Bioscience

Services

Australian Cancer Research Foundation's (ACRF) Cancer Biology Imaging Facility has imaging facilities and screening of chemical and biological libraries. Capabilities of the facility include laser scanning and spinning disc confocal microscopy, deconvolution, high-throughput multi-wall imaging, and 3D optical projection tomography (OPT). It is home to 23 high-performance microscopes and supporting image data analysis workstations, with facility staff on-site to provide users with expert technical support and training.

Equipment

N/A

Contact

Doctor James Springfield

Institute for Molecular Bioscience

(07) 3346 2390

Australian Cancer Research Foundation (ACRF) Cancer Biology Imaging Facility
Institute of Molecular Bioscience (Building 76), St Lucia campus

Centre for Microscopy and Microanalysis

Services

The Centre for Microscopy and Microanalysis is well-equipped with a suite of analytical instruments for research in the physical and biological sciences.

Equipment

  • JEOL 1010 Transmission Electron Microscope
  • JEOL 1011 Transmission Electron Microscope
  • Philips TECNAI 12 Transmission Electron Microscope
  • Philips TECNAI 20 Transmission Electron Microscope
  • JEOL 2100 Transmission Electron Microscope
  • Philips TECNAI 30 Transmission Electron Microscope
  • JEOL JCM-5000 Scanning Electron Microscope
  • Hitachi SU3500 Scanning Electron Microscope 
  • Philips XL30  SEM with a LaB6 source electron gun
  • JEOL JSM-6460LA variable pressure Scanning Electron Microscope
  • JEOL JSM-6610 high resolution, analytical Scanning Electron Microscope
  • JEOL JSM-7001F Field Emission Scanning Electron Microscope
  • JEOL JSM-7100F Field Emission Scanning Electron Microscope
  • Zeiss Sigma with Gatan 3View Serial Blockface Scanning Electron Microscopy
  • JEOL JSM-7800F Field Emission Scanning Electron Microscope
  • JEOL JXA-8200 EPMA (Probe) 
  • FEI - SCIOS FIB analytical DualBeam™ system
  • SAXS Small-angle X-ray scattering
  • XPS - X-ray Photoelectron Spectroscopy
  • Powder X-Ray Diffraction
  • Rigaku Thin-Film X-Ray Diffraction and Reflectometry

Contact

Centre for Microscopy and Microanalysis

(07) 3346 3944

Level 1, AIBN Building 75, St Lucia campus

Cryo-EM - Centre for Microscopy and Microanalysis

Services

Purpose-built for standard and cryo-TEM sample preparation and analysis, as well as electron tomography of both resin-embedded and cryo-samples (for three-dimensional analysis).

Equipment

  • Tecnai F30 300keV Field Emission Gun (FEG) TEM with a 4k Direct Electron LC1100 (lens-coupled) Camera
  • Tecnai T12 120keV TEM, with a 4k FEI Eagle CCD Camera  
  • JEOL 1011 100 keV  TEM with an Olympus SIS Morada CCD Camera
  • JEOL JCM5000 Benchtop SEM (Neoscope)
  • Two high-pressure freezer systems: BalTec HPM010 and Leica EM PACT2
  • Leica AFS and EM-AFS2 (automatic freeze substitution systems) with an automated freeze substitution processor
  • PELCO Biowave microwave
  • FEI Vitrobot
  • Leica EM UC6 ultramicrotomes and cryo-ultramicrotomes
  • Nikon Eclipse Ti-E fluorescence microscope
  • Tousimis Auto Critical Point Dryer

Contact

Centre for Microscopy and Microanalysis

(07) 3346 3944

Queensland Bioscience Precinct - Cryo-TEM Facility
Molecular Biosciences Building (Building 76), St Lucia campus

Microscopy - Centre for Advanced Light Microscopy (School of Biomedical Sciences)

Services

Self and full service:

  • Confocal microscopy
  • Multiphoton microscopy
  • Digital deconvolution
  • Epifluorescence microscopy
  • Automated data analysis
  • Image analysis functions development

Equipment

  • Zeiss LSM 510 META Confocal Microscope
  • Olympus BX61 Upright Confocal Microscope
  • Aperio slide scanner
  • Stereology microscope

Contact

Doctor Shaun Walters

School of Biomedical Sciences

(07) 3365 3606

School of Biomedical Sciences, St Lucia campus

Microscopy - Queensland Brain Institute

Services

  • super-resolution imaging (SIM, STORM/PALM)
  • automated imaging/Slide scanning
  • optical sectioning
  • tiled imaging of large areas 
  • neuronal morphology analysis
  • high-speed live imaging
  • light sheet microscopy (SPIM)
  • stereology 
  • calcium imaging
  • total internal reflection fluorescence (TIRF)
  • FLIM
  • FRET (Förster resonance energy transfer)
  • FRAP (Fluorescence recovery after photobleaching)
  • 2-photon imaging

Equipment

  • Zeiss LSM710 Confocal scanning microscopes
  • Zeiss LSM510 META Confocal Microscope
  • 3i Yokogawa W1 SDC spinning disk confocal microscope
  • Andor Dsikovery spinning disk confocal microscope
  • Custom-built light-sheet microscope (SPIM)
  • Zeiss ELYRA PS super-resolution microscope
  • Roper Ilas2 dual laser illuminator (TIRF)

Contact

Microscopy Facility Manager

Rumelo Amor

Queensland Brain Institute

(07) 3346 6300

Queensland Brain Institute (Building 79), St Lucia campus

Microscopy - UQ Diamantina Institute

Services

Fully equipped microscopy suite, with computers containing a range of analysis software for analytical purposes.

Equipment

The following equipment is available to UQ users as part of the 'Core Facilities at TRI'.

  • Zeiss LSM 510 Meta Confocal
  • Zeiss Live Cell Imager
  • LaVision Biotec Multiphoton microscope
  • Nikon Brightfield microscope with camera
  • Olympus FV 1200 confocal*
  • Nikon/Spectral Spinning disc confocal microscope*
  • GE Healthcare 3D-SIM OMX Braze super resolution microscope*
  • Olympus excellence live cell imaging system*
  • Epifluorescent inverted microscopes (X4)
  • Olympus Slide Scanner Brightfield*
  • Olympus BX63F motorized upright fluorescence microscope*
  • IX73 inverted fluorescence microscopes
  • Olympus VS120 Brightfield slide scanner

(* TRI owned)

Contact

Doctor Sandrine Roy

Translational Research Institute (TRI)

TRI Building (Building 48), Princess Alexandria Hospital campus

Optical microscopy - School of Chemistry and Molecular Biosciences

Services

A research technique in optical microscopy that relies on excitation of fluorescent molecules with a specific wavelength region to produce an image generated by the secondary fluorescence emission at longer wavelengths. Modern fluorescence microscopes are equipped with reflected light illuminators that incorporate neutral density filters and a specialized combination of interference filters to segregate incident illumination from the detected fluorescence emission.  Confocal laser-scanning fluorescence microscopy can be used to determine the localisation of molecules eg protein or mRNA in live or fixed cells and tissues.  This means one can follow transport of protein- or RNA-containing granules in cells.

Equipment

  • ZEISS LSM510 META Upright Confocal Microscope with AxioCam MRm camera
  • ZEISS LSM710 Inverted Confocal Microscope with environmental chamber
  • GE InCell Analyser 1000 HCA Imaging System for live and fixed cells.
  • ZEISS Axioplan 2 epifluorescent/light microscope with high-resolution AxioCam 506 colour digital camera camera
  • Nikon Axiophot epifluorescent/light microscope with Spot digital colour camera
  • Dedicated workstations for Image visualisation, manipulation and analysis.
  • Nikon TS100 inverted bright-field and epi-fluorescent microscope with imaging system.
  • Nikon Ts2 inverted bright-field microscope with colour imaging system.
  • Daito XP9000 Automatic X-Ray Film Developer
  • UV Gel-documentation System with instant high-gloss thermal printout

Contact

Doctor Steven Mason

School of Chemistry and Molecular Biosciences

(07) 3365 4872

Microscopy and Imaging Facility (School of Chemistry and Molecular Bioscience)
Rooms 433 and 409, Molecular Biosciences Building (Building 76), St Lucia campus